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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH IF

Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H1 is located in the nuclear and is necessary for the condensation of nucleosome chains into higher order structures. Histone H1 produces a speckled staining pattern in the nucleus of normal and malignant cells.
Catalog #	H5300-10
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunoperoxidase Staining and Immunofluorescence. Other applications have not been tested.
Recommended Dilutions	ELISA: 1:1000 when histones are immobilized on polyvinyl chloride plates.
	Western Blot: 1:500 detects Histone H1 and core proteins on 10ug of Jurkat lysates.
	Immunoperoxidase Staining: 1:500, cold methanol fixation. Immunofluorescence: 1:500, cold methanol fixation.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
	I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Positive Control	NIH3T3 and HeLa nuclear extract
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	2Q2205
Host	Mouse
Concentration	Not Determined
Form	Supplied as a liquid. No preservatives added.
Purity	Ascites
Immunogen	Histones purified from calf thymus. MW: 33kD.
Specificity	Recognizes human Histones, H1 and core proteins H2a, H2b, H3, H4. Species Sequence Homology: bovine, mouse and hamster.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.