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You are here:Home » Antibodies » Abs to Histones » Anti -Histones

Anti -Histones


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H1 is located in the nuclear and is necessary for the condensation of nucleosome chains into higher order structures. Histone H1 produces a speckled staining pattern in the nucleus of normal and malignant cells.
Catalog #H5300-10
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5ug/ml detects Histone H1 and core proteins in10ug of Jurkat cell lysate.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Positive ControlJurkat cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight~14-33kD (observed)
Clone TypeMonoclonal
Clone No2Q2205
FormSupplied as a liquid in TGS buffer (pH 7.4), with 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHistones purified from calf thymus. MW: 33kD.
SpecificityRecognizes human Histones, H1 and core proteins H2a, H2b, H3, H4. Species Sequence Homology: bovine, mouse and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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