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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH IC

There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure.
Catalog #	H5305
Applications	Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry: 1-5ug/ml
	Western Blot: Detects a band at ~20kD
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q2283
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide
Purity	Purified by Protein A affinity chromatography.
Immunogen	Purified nuclear fractions of HeLa cells
Specificity	Binds specifically to an antigen determinant, which is present on all five histone proteins. Recognizes the histones H1, H2A, H2B, H3 and H4 with comparable sensitivity.
Species Crossreactivity	Human, bovine, rat, Xenopus, opossum and hamster.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.