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You are here:Home » Antibodies » Abs to Histones » Anti -Histones, pan

Anti -Histones, pan

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure.
Catalog #H5305
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 1-5ug/ml
Western Blot: Detects a band at ~20kD
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q2283
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide
PurityPurified by Protein A affinity chromatography.
ImmunogenPurified nuclear fractions of HeLa cells
SpecificityRecognizes human histones H1, H2A, H2B, H3 and H4 with comparable sensitivity. Binds specifically to an antigen determinant, which is present on all five histone proteins.
Species CrossreactivityBovine, rat, Xenopus, opossum and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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