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You are here:Home » Antibodies » Abs to Infectious Disease HIV » Anti -HIV-1 p24, Core (Human Immunodeficiency Virus-1)

Anti -HIV-1 p24, Core (Human Immunodeficiency Virus-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. Structural studies demonstrated two distinct domains (N and C-terminal domains) that have different roles in the virus morphogenesis. Additionally, the level of p24 found in the blood serum is used as an indicator of HIV progression. HIV-1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
Catalog #H6003-25M
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA (Capture): 4ug/ml. Coat 100ul/well with TMB substrate.
Western Blot: 0.1ug/ml antibody with 0.1ug /well of Recombinant HIV P24.
Optimal dilutions to be determined by the researcher.
Matched PairCapture: H6003-25M
Dectection: H6003-01T
HybridomaSp2/0-AG14 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No9A96
HostMouse
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 500ul sterile ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to HIV-1 P24
SpecificityRecognizes HIV-1 P24 (core protein). Does not react with HIV-1 GP41 or HIV-2 GP36.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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