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You are here:Home » Antibodies » Abs to Infectious Disease HIV » Anti -HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (HRP)

Anti -HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (HRP)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E
Catalog #H6003-34F1
Molar Ratio2-4 peroxidase molecules per molecule of mAb.
Biological ActivityPeroxidase conjugated Murine anti-gp120 mAb binds to gp120 in CD4/gp120 capture ELISA and Western ELISA.
ApplicationsA dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1 : 5000 depending on the concentration of gp120 in the test system. CD4/gp120 capture ELISA may be performed in 1 : 500 dilution range depending on the capture ligand CD4 concentration and gp120 concentration in the test sample. Typical assays with 100ng of solid phase CD4 will detect at least 10ng/ml gp120.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No5i18
HostMouse
ConcentrationAs reported
FormSupplied as a liquid in 10mM Tris, pH 8.0, 1% BSA, 0.01% Thimerasol. Conjugated to HRP.
Purity95%
SpecificityBinds to native and recombinant gp120 IIIB (V3 loop specific) in ELISA and Western ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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