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You are here:Home » Antibodies » Abs to Infectious Disease HIV » Anti -HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (FITC)

Anti -HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified IH FC IF Gel
HIV is a member of the genus Lentivirus, part of the family of Retroviridae, which use reverse transcriptase to convert the viral RNA genome into double stranded DNA. This is then integrated into the cellís DNA, which is followed by latency or active HIV replication. HIV primarily infects CD4+ T lymphocytes, resulting in their dramatic decline. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. If untreated, this can lead to AIDS (Acquired Immunodeficiency Syndrome). Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.
Catalog #H6003-34F3
ApplicationsSuitable for use in Dot Blot, FACS, Immunohistochemistry and Direct Immunofluorescence. Other applications not tested.
Recommended DilutionsDirect Immunofluorescence: 1:50
Dot Blot: 1:200
Optimal dilutions to be determined by the researcher.
F/P Ratio3-4
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG
Clone No5i20
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in 10mM Tris, pH 8.0. No stabilizers or preservatives added. Labeled with fluorescein isothiocyanate (FITC).
Purity95% Purified by gel exclusion chromatography.
ImmunogenHIV-1 gp120
SpecificityRecognizes native and recombinant HIV-1 gp120.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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