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You are here:Home » Antibodies » Abs to Infectious Disease HIV » Anti -HIV-1 gp120 (Biotin) (Human Immunodeficiency Virus Type 1)

Anti -HIV-1 gp120 (Biotin) (Human Immunodeficiency Virus Type 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E B IC FC
Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.
Catalog #H6003-34G2
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and FACS. Other applications not tested.
Recommended DilutionELISA: 1:5000
Western Blot: 0.1-0.5ug/ml
Optimal dilutions to be determined by the researcher.
Biotin/Protein ratio 10-20
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No5i24
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 2.5% BSA, 0.02% sodium azide. Labeled with Biotin.
PurityPurified by gel exclusion chromatography. ( 95% pure).
ImmunogenHIV-1 gp120 (Human Immunodeficiency Virus Type 1)
SpecificityRecognizes native and recombinant HIV-1 gp120.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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