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You are here:Home » Antibodies » Abs to Infectious Disease HIV » Anti -HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1)

Anti -HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH N
HIV-1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
Catalog #H6003-34H
ApplicationsSuitable for use in ELISA, Western Blot, Neutralization, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
ELISA: Yields titers of >1:2000 with 100ng of immobilized gp120 under standard ELISA conditions.
Western Blot: 1-2ug/ml depending on the nature and quantity of the immobilized antigen.
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.
Biological ActivityNeutralizes HIV-1 as determined by syncycium assays using infected CD4 bearing cells and human PBL co-cultures.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
PurityPurified by Protein A affinity chromatography ( 95% SDS-PAGE, reduced)
ImmunogenHIV-1 gp120
SpecificityRecognizes native and recombinant HIV-1 gp120/gp160 in ELISA and Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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