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You are here:Home » Antibodies » Abs to Infectious Disease HIV » Anti -HIV-1 gp120 (Human Immunodeficiency Virus-1)

Anti -HIV-1 gp120 (Human Immunodeficiency Virus-1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH N
Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.
Catalog #H6003-34K
ApplicationsSuitable for use in ELISA, Western Blot, Neutralization, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsELISA: 1:2000 with using 100ng of immobilized gp120 coated on the plate.
Western Blot: 1-2ug/ml
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.
Biological ActivityNeutralizes HIV-1 as determined by syncycium assays using infected CD4 bearing cells and human PBL co-cultures.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
PurityPurified by Protein A affinity chromatography to 95% as determined by SDS-PAGE, reduced.
ImmunogenRecombinant HIV-1 gp120.
SpecificityRecognizes native and recombinant HIV-1 gp120/gp160. Does not cross react with other human or animal proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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