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You are here:Home » Kits and Assays » Kits for ELISA (Infectious Disease), BioAssay™ » HIV-1, p24 (Human Immunodeficiency Virus 1), ELISA Kit, BioAssay™

HIV-1, p24 (Human Immunodeficiency Virus 1), ELISA Kit, BioAssay™

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Specifications

Catalog #H6005-10P
Principle of the AssayMicrotitration wells coated with murine anti-HIV-1 P24 capture antibody, are exposed to test specimens, which may contain p24 reactive determinants. After an incubation period, unbound components in the test sample are washed away. Specifically bound p24 reactive determinants react with a mouse anti-HIV-1 p24 conjugated with biotin during a second incubation period. Following a second wash cycle the biotinylated antibody is detected by the addition of a streptavidin HRP conjugate. Following a third wash cycle, specifically bound enzyme conjugate is detected by reaction with the Substrate solution, tetramethylbenzidine (TMB). The assay is measured spectrophotometrically to indicate the level of p24 reactive determinants present in a sample.
Qualitative AnalysisNegative control should be 0.10
100pg/ml control should be 0.60
Sensitivity1.7pg/ml.
Kit ComponentsH6005-10P1: Microtiter Strips, 1x96 wells
Plastic microtitration wells coated with anti-HIV-1 p24 murine monoclonal antibody in foil pouch with desiccant.
H6005-10P2: Positive Control 10ng/ml, 1x100ul
H6005-10P3: Lysis Buffer, 1x6ml
*H6005-10P4: Antibody (Biotin), 1x12ml
H6005-10P5: Streptavidin (HRP), 1x12ml
Containing 0.01% Bromonitrodioxane as preservative.
H6005-10P6: Wash Buffer 20x, 2x60ml
Tris buffered saline pH 7.8-8.0, containing 0.05% Tween 20. Must be diluted before use.
H6005-10P7: Substrate (TMB) Solution, 1x12ml
Ready to use.
H6005-10P8: Stop Solution. 1N H2SO4, 1x12ml
Storage and StabilityAliquot and store *H6005-10P4 at -20°C. Store other components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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