Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:3000 Immunoprecipitation: 1:750 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HIV-1 tat-Glutathione-S-transferase (GST) fusion protein in E. coli.
Form
Supplied as a liquid. Preservative free.
Specificity
Recognizes wild type HIV-TAT protein. Antibody specificity was analyzed by Western Blot with control GST-tat fusion protein (MW 37kD) and detection using the ECL system. Crossreacts with other GST fusion proteins unless pre-adsorbed against GST.
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