| The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. |
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| Catalog # | H6098-07B |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: 10ug/ml. Use 10ul to label 10e6 cells or 100ul whole blood. |
| Immunohistochemistry: frozen |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents (FC) | F(ab')2 rabbit anti-mouse IgG FITC, |
| F(ab')2 rabbit anti mouse IgG: RPE |
| Recommended Secondary Reagents (IHC) | F(ab')2 rabbit anti-mouse IgG HRP |
| Recommended Positive Control Tissue (IHC) | Tonsil. |
| Recommended Negative Controls (IHC) | Mouse IgG2b Negative Control |
| Hybridoma | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 3H2205 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Papain solubilised HLA-A2. |
| Specificity | Recognizes the human HLA-A2 histocompatibility antigen. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
