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Anti -HLA Class 1 Antigen A2 (IgM, R0.98)
Anti -HLA Class 1 Antigen A2 (IgM, R0.98)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
FC IF |
|
| Catalog # | H6098-08 | | Applications | Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies. Other applications have not been tested. | | Recommended Dilution | Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution. | | Optimal dilutions to be determined by the researcher. | | Cytotoxicity | Expected Results: | | Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. | | Limitations: | | Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results. | | Source | Ascites fluid | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Please contact United States Biological for bulk requirements and licensing requests relating to production applications. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 4i74 | | Host | Mouse | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution. | | Purity | Ascites | | Immunogen | B cells expressing HLA class I antigen | | Specificity | Reacts to HLA Class 1 Antigen-A2. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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