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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen A33,B8

Anti -HLA Class 1 Antigen A33,B8

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites
The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans and is an essential element for immune function. This group of genes re- sides on chromosome 6, and encodes cell-surface antigen- presenting proteins and many other genes. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell, including viral peptides if present. These peptides are produced from digested proteins that are broken down in the proteasomes. Foreign antigens attract killer T-cells (also called CD8+ or cytotoxic T-cells) that destroy cells.
Catalog #H6098-39
ApplicationsThis antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
Recommended DilutionCytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution.
Optimal dilutions to be determined by the researcher.
CytotoxicityExpected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Recon- stituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No4i104
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
PurityAscites
SpecificityRecognizes HLA Class 1 Antigen A33,B8. Specificity was determined at a 1:10 dilution by the Microcytotoxicity Assay under standard NIH conditions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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