| Catalog # | H6098-39F |
| Applications | Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation. |
| Recommended Dilution | Flow Cytometry: 1:50-1:100 |
| Immunohistochemistry: Frozen |
| Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. |
| Optimal working dilutions to be determined by researcher. |
| Recommended Positive Control | Tonsil |
| Recommended Negative Controls | Mouse IgG2a Negative Control |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H2211 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Membrane of human tonsil cells. |
| Specificity | Recognizes an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognizes virtually all nucleated human cells. Species Crossreactivity: Clone is reported to crossreact with Rhesus macaque (4), bovine (4), feline (5), Cynomolgus monkey (6) and baboon (7). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
