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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen ABC

Anti -HLA Class 1 Antigen ABC

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
Catalog #H6098-39F
Applications Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation.
Recommended DilutionFlow Cytometry: 1:50-1:100
Immunohistochemistry: Frozen
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.
Optimal working dilutions to be determined by researcher.
Recommended Positive ControlTonsil
Recommended Negative ControlsMouse IgG2a Negative Control
Recommended Secondary ReagentsF(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate
Fusion PartnersSpleen cells from immunized BALB/c mice were fused with cells of the mouse NS1/1-Ag4.1 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2211
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenMembrane of human tonsil cells.
SpecificityRecognizes an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognizes virtually all nucleated human cells. Species Crossreactivity: Clone is reported to crossreact with Rhesus macaque (4), bovine (4), feline (5), Cynomolgus monkey (6) and baboon (7).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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