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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen ABC (Biotin)

Anti -HLA Class 1 Antigen ABC (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Catalog #H6098-39F2
ApplicationsSuitable for use in Flow Cytometry. Other applications have not been tested.
Recommended DilutionFlow Cytometry: Neat-1:5. Use 20ul to label 10e6 cells or 100ul whole blood.
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsS7973-78: Streptavidin (PE)
S7973-92F2: Streptavidin (PE)
S7973-96A: Streptavidin (FITC)
S7973-96B: Streptavidin (FITC)
Recommended Negative ControlsI1904-83V1: IgG2a Negative Control (Biotin)
HybridomaSpleen cells from immunized BALB/c mice fused with mouse NS1/1-Ag4.1 myeloma cells
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2211
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human tonsil lymphocyte membranes.
SpecificityRecognizes an antigenic determinant shared among products of the HLA A, B and C loci. Recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains. Recognizes virtually all nucleated human cells. Species Crossreactivity: Rhesus macaque, bovine, feline, Cynomolgus monkey and baboon.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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