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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen ABC

Anti -HLA Class 1 Antigen ABC

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP IH FC IF
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Catalog #H6098-39F5
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:100. 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections
Optimal dilution determined by the researcher.
HybridomaSpleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6A409
HostRat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPHA activated human peripheral blood lymphocytes.
SpecificityRecognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. May be able to induce apoptosis in activated but not resting T cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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