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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified IP IH FC IF

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.
Catalog #	H6098-39F5
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:50-1:100. 10ul labels 10e6 cells in 100ul.
	Immunohistochemistry: Frozen sections
	Optimal dilution determined by the researcher.
Hybridoma	Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	6A409
Host	Rat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	PHA activated human peripheral blood lymphocytes.
Specificity	Recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. May be able to induce apoptosis in activated but not resting T cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.