| MHC class I glycoproteins are found on the surface of almost all nucleated somatic cells. |
|
| Catalog # | H6098-39J |
| Applications | Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul |
| Immunohistochemistry: frozen sections |
| Optimal dilutions to be determined by the researcher. |
| Histology Positive Control Tissue | Tonsil |
| Hybridoma | NS1/1-Ag4.1 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 10D170 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4. No preservative added. Endotoxin Level: <0.01 EU/ug |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Purified human tonsil lymphocyte membranes |
| Specificity | Recognizes an antigenic determinant shared among products of the human HLA A, B and C loci. Recognizes a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains (6) |
| Species crossreactivity | Macaque, bovine, feline, Cynomolgus monkey, Baboon, Rhesus Monkey, Chimpanzee, Gorilla, Shrew. Does not react with goat, canine, guinea pig, rabbit, mouse, chicken or amphibia. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | HLA Class 1 A, HLA A, HLA Class 1 B, HLA B, HLA Class 1 C, HLA C, MHC Class I HLA A, MHC Class I HLA B, MHC Class I HLA C |
