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You are here:Home » Antibodies » Antibodies-HLA » Anti -HLA Class 1 Antigen ABC (HLA ABC, Major Histocompatibility Complex Class I A + B + C, MHC HLA ABC)

Anti -HLA Class 1 Antigen ABC (HLA ABC, Major Histocompatibility Complex Class I A + B +


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
MHC class I antigens associated with B2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.
Catalog #H6098-39R
ApplicationsSuitable for use in Blocking (6,7), Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation (2), Inhibition of NK cell-mediated lysis (10), and Activation (8,9). Other applications not tested.
Recommended DilutionsImmunofluorescence: 2ug/10e6 cells in 100ul volume or 100ul whole blood.
Western Blot: Non-reducing (3)
Immunohistochemistry: Acetone-fixed frozen sections (4,5)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoW6/32
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by affinity chromatography.
SpecificityRecognizes human HLA-A,B,C. Recognizes a non-polymorphic epitope. Species Crossreactivity: chimpanzee, baboon, Cynomolgus monkey, Rhesus monkey, bovine and feline.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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