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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen B7 (PE)

Anti -HLA Class 1 Antigen B7 (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.
Catalog #H6098-43B
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: Neat. Use 10ul to label 1x10e6 cells or 100ul whole blood.
Optimal working dilutions to be determined by researcher.
Recommended Negative ControlsI1904-79D: IgG1 Murine Negative Control (PE)
Storage and StabilityLyophilized powder may be stored at 4°C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2215
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenPapain solubilized HLA-B7 antigen.
SpecificityRecognizes human HLA-B7 antigen. Does not crossreact with HLA-B27 or other related HLA antigens. Clone 3H2215 can be used to distinguish true HLA-B27 positives from HLA-B27 false positives (ie HLA-B7 positive) in the investigation of such diseases as ankylosing spondylitis and anterior uveitis. Species Crossreactivity: Cynomolgus monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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