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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites FC IF

Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Catalog #	H6098-48
	Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Applications	Suitable for use in Cell Typing, Tissue Staining, and Chimerism Studies. Other applications not tested.
Recommended Dilutions	For a cytotoxic working dilution, add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution. Store aliquots at -20°C.
Expected Results	Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations	Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	4i109
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
Purity	Ascites
Immunogen	HLA Class 1 Antigen-B13, B62, B15.
Specificity	Recognizes HLA Class 1 Antigen-B13, B62, B15. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.