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Anti -HLA Class 1 Antigen B17,B35,B44(X-Reactive)
Anti -HLA Class 1 Antigen B17,B35,B44(X-Reactive)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
FC IF |
|
| HLA CLass I is involved in the presentation of foreign antigens to the immune system. This antibody is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. | | | Catalog # | H6098-50 | | Applications | Animal Cell Typing, Tissue Staining, and Chimerism Studies. Please refer to the biotinylated versions of selected antibodies for Flow Cytometry applications. FITC-conjugated and purified versions of these antibodies are available on special order. | | Directions for Use | For a cytotoxic working dilution, add 0.9ml of 1% BSA in PBS to 100ul of10X working stock. Store aliquots at -20C. | | Expected Values | Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. | | Limitations | Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 4i111 | | Host | Mouse | | Source | Human | | Concentration | Not Determined | | Form | Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution. | | Purity | Ascites | | Specificity | Recognizes human HLA Class 1Antigen-B17,B35,B44. Additional cross reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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