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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen B27 (FITC)

Anti -HLA Class 1 Antigen B27 (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified FC
This is a cytotoxic antibody which detects HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27+ and precipitates cell surface molecules of 43 and 12kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
Catalog #H6098-52D
ApplicationsSuitable for use in Flow Cytometry. Other applications have not been tested.
Recommended DilutionsFlow Cytometry: Neat; Use 10ul to label 10e6 cells or 100ul of whole blood.
Optimal working dilutions to be determined by researcher.
Recommended Negative ControlI1904-78R3: IgG1 Murine Negative Control (FITC)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2219
HostMouse
SourceHuman
Concentration~0.05mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Ion Exchange chromatography.
ImmunogenImmune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
SpecificityRecognizes HLA Class 1 Antigen B27. Affinity studies by Scatchard analysis showed that HLA-B27 antibody has a higher affinity for HLA-B27 (9.7 x 10e8M-1) than for HLA B7 (9.5 x 10e7 M-1). Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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