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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive)

Anti -HLA Class 1 Antigen B35,B57,B75,B77(X-Reactive)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites
Catalog #H6098-55
ApplicationsThis antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
Recommended DilutionsCytotoxic Working Dilution: Add 0.9ml 1% BSA in PBS to 100ul of10X stock solution.
Optimal dilutions to be determined by the researcher.
Expected ResultsCell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
LimitationsCell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No4i116
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyphilized powder. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
PurityAscites
ImmunogenB cells expressing HLA class I antigen
SpecificityRecognizes HLA Class 1 Antigen-B35,B57,B75,B77. Additional crossreactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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