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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen ABC (HLA ABC, Major Histocompatibility Complex Class I A + B + C, MHC HLA ABC) (PerCP)

Anti -HLA Class 1 Antigen ABC (HLA ABC, Major Histocompatibility Complex Class I A + B +
C, MHC HLA ABC) (PerCP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
HLA-A, B, and C are ~45kD transmembrane glycoproteins in the major histocompatibility complex I (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12kD beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.
Catalog #H6098-60D
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of conjugated antibody was added to 1- 2.5x10e5 cells and incubated for 30 minutes at RT.
3. Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PerCP-labeled mouse IgG2A antibody.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2A
Clone NoW6/32
HostMouse
SourceHuman
Concentration~0.025mg/ml
FormSupplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with PerCP.
PurityPurified by Protein A affinity chromatography.
ImmunogenMembranes from human tonsillar lymphocytes (not from a recombinant protein).
SpecificityRecognizes human MHC class I, HLA-A, B, and C.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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