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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified FC IF

Catalog #	H6098-62A
Applications	Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies. Other applications have not been tested.
Recommended Dilutions	Flow Cytometry: 10ul/test
	Optimal dilutions to be determined by the researcher.
Cytotoxicity	Expected Results:
	Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
	Limitations:
	Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	0.L.5
Host	Mouse
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid in PBS, 0.1% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (FITC).
Purity	98%, Purified by Ion Exchange chromatography.
Specificity	Recognizes HLA Class 1 Antigen-Bw6.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.