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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen

Anti -HLA Class 1 Antigen

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP FC
Catalog #H6098-65E
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L252
HostMouse
ConcentrationAs reported
Form15mM Sodium Azide; PBS. pH 7.4
PurityPurified by immunoaffinity chromatography.
ImmunogenPHA-activated peripheral blood lymphocytes.
SpecificityReacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). Positive in Western blotting (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesHLA-A, HLA-B, HLA-C, HLA-F, HLA-G


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