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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen, monomorphic

Anti -HLA Class 1 Antigen, monomorphic

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
The class I major histocompatibility complex (or human leukocyte antigen) monomorphic antigen is expressed widely on human nucleated cells such as lymphocytes, monocytes/ macrophages, dendritic cells and other hematopoietic and non-hematopoietic nucleated cells, and platelets. The expression of HLA-I antigen varies with cell types and decreases with cell maturation, such as less expression on mature granulocytes and almost losing on mature erythrocytes. HLA-I antigen is also expressed on most cells of human leukemic cell lines, but not or little on the cells of Daudi and K562 cell lines and is absent from nerve cells. In addition to membrane HLA-I antigen, soluble form of HLA-I can be detected in serum (or plasma).
Catalog #H6098-67
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recomended DilutionsFlow Cytometry: 0.06ug labels 100ul peripheral blood.
Immunohistochemistry: Acetone fixed frozen and formalin-fixed paraffin sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F61
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes human monomorphic HLA Class 1 Antigen. In addition to membrane HLA-I antigen, the soluble form of HLA-I can be detected in serum (or plasma). Reacts specifically with normal human peripheral blood lymphocytes (100%) and monocytes (96%). Does not react with normal human peripheral blood granulocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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