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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen, monomorphic (PE/Cy5)

Anti -HLA Class 1 Antigen, monomorphic (PE/Cy5)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The expression of HLA-I antigen varies with cell types and decreases with cell maturation, such as less expression on mature granulocytes and almost losing on mature erythrocytes. HLA-I antigen is also expressed on most cells of human leukemic cell lines, but not or little on the cells of Daudi and K562 cell lines and is absent from nerve cells. In addition to membrane HLA-I antigen, soluble form of HLA-I can be detected in serum (or plasma).
Catalog #H6098-67C
ApplicationsSuitable for use in flow cytometric analysis using 100ul peripheral blood cells or 10ul/10e6 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F61
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Conjugated to PE/Cy5.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes class I major histocompatibility complex (or human leukocyte antigen) monomorphic antigen which expresses widely on human nucleated cells such as lymphocytes, monocytes/ macrophages, dendritic cells and other hematopoietic and non-hematopoietic nucleated cells, and platelets.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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