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You are here:Home » Antibodies » Antibodies-HLA » Anti -HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E, HLA-6.2, HLAE)

Anti -HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E,
HLA-6.2, HLAE)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
Catalog #H6098-68F
HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia) HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
ApplicationsSuitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunohistochemistry (FFPE): 10ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with H6098-68F, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone NoMEM-E/07
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to HLA-E refolded with beta2-microglobulin and peptide.
SpecificityRecognizes native surface-expressed human HLA-E, but not denatured heavy chain of HLA-E.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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