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Anti -HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E, HLA-6.2, HLAE)
Anti -HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E, MHC Class I Antigen E,
HLA-6.2, HLAE)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP IH FC |
|
| Catalog # | H6098-68F | | HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia) | HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly). | | Applications | Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (FFPE): 10ug/ml. Validated for use on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with H6098-68F, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | MEM-E/07 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Recombinant protein corresponding to HLA-E refolded with beta2-microglobulin and peptide. | | Specificity | Recognizes native surface-expressed human HLA-E, but not denatured heavy chain of HLA-E. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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