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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 1 Antigen G (FITC)

Anti -HLA Class 1 Antigen G (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified IP IH IC FC
HLA-G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells
Catalog #H6098-72M8
ApplicationsSuitable for use in FLISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:500
FLISA: 1:2000
Immunocytochemistry: 5ug/ml
Immunohistochemistry (Frozen): 1:200
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8L266
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with FITC.
PurityPurified
ImmunogenRecombinant human HLA-G refolded with beta2-microglobulin and peptide. (NM_002127). Cellular Localization: Cell Membrane
SpecificityRecognizes the native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. Does not react with mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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