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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 2 Antigen DP

Anti -HLA Class 2 Antigen DP

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Specifications

Clone Host Grade Applications
Mouse Purified E B IP IH FC
HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules. Can be used for tissue typing on lymphoblastoid cells, transplantation typing, disease susceptibility and detection of class II products.
Catalog #H6099-84B
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:100-1:2000
Western Blot: 1:100:1:2000
Immunohistochemistry (Frozen): 1:10-1:2000
Flow Cytometry: 1:10-1:1000
Immunoprecipitation: 1:10-1:500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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