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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 2 Antigen DP

Anti -HLA Class 2 Antigen DP

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
DP monomorphic antigen is expressed on hematopoietic progenitors, B lymphocytes, monocytes/ macrophages, dendritic cells, langerhans cells, activated T cells, some endothelial and epithelial cells, but not on resting T lymphocytes, erythrocytes and platelets. HLA-DP antigen is also expressed at high levels on B lymphocyte leukemic cells and cells of CML-LBC, at moderate levels on the cells of AML-M1/M2, M4 and M5, but not or little on the cells of AML-M3 and CML. This antibody might be used as valuable reagent in diagnosis and classification of leukemias.
Catalog #H6099-84E
ApplicationsSuitable for use in indirect immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.015ug/1x10e6 cells or 100ul peripheral blood.
Immunohistochemistry: Acetone-fixed frozen sections and formalin-fixed paraffin sections.
Optimal dilution determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F63
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.0, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes normal human peripheral blood lymphocytes (25%) and monocytes (95%). Does not recognize normal human periphral blood granlocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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