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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 2 Antigen DR (SLA Class II DR) (FITC)

Anti -HLA Class 2 Antigen DR (SLA Class II DR) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In pigs, this is referred to as the swine leukocyte antigen (SLA) region. There are 3 major MHC class II proteins encoded by the SLA which are SLA DP, SLA DQ and SLA DR.
Catalog #H6099-95Z1
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
HybridomaX63-Ag.8.653 myeloma cells with spleen cells from Balb/c mice.
Recommended Negative ControlI1904-84U3: IgG2b Negative Control (FITC)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No10D419
HostMouse
SourcePorcine
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenPorcine monocytes
SpecificityRecognizes porcine HLA Class 2 Antigen DR (SLA-II DR) expressed on all B cells, antigen presenting cells and on certain subsets of resting and activated T cells. Reacts with lymphocytes from outbred and miniature pigs, suggesting a reaction with a monomorphic determinant. Immunoprecipitates a heterodimer composed of two polypeptides of 28 and 35kD from NP-40 extracts of biotin surface-labelled porcine peripheral blood mononuclear cells. Inhibits the mixed lymphocyte reaction and T cell stimulation induced by African swine fever virus and staphylococcal enterotoxin B. Species Crossreactivity: bovine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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