Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:10-1:1000 ELISA: 1:100-1:2000 Western Blot: 1:100-1:2000 Immunohistochemistry (Frozen): 1:10-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to HLA Class 2 Antigen DR beta from the membranes of LKT cells (a B lymphoblastoid cell line). |Cellular Localization: Cell Membrane
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity
Purified
Specificity
Recognizes HLA-DR beta chain 1. Reacts with all DR and DP except DR7. Not specific to humans.
References
1.Brodsky FM et al. Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens 16:30-48 (1980). <PUBMED:6162228>Brodsky FM et al. Monoclonal antibodies for analysis of the HLA system. Immunol Rev 47:3-61 (1979).
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