| Catalog # | H6099-97 |
| Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: Neat-1:10. Use 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry: Frozen, Paraffin. |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents (Flow Cytometry) | F(ab')2 Goat anti-rat IgG: FITC (mouse adsorbed), F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed) |
| Recommended Secondary Reagents (Immunohistochemistry) | Goat anti-rat IgG: HRP (mouse adsorbed) |
| Recommended Negative Controls | Rat IgG2a Negative Control (for human) |
| Rat IgG2a Negative Control (for mouse) |
| Hybridoma | Spleen cells from immunized AO rats were fused with cells of the rat Y3 Ag1.2.3 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H2232 |
| Host | Rat |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 0.2M Tris HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide. |
| Purity | Tissue Culture Supernatant. |
| Immunogen | EHR-B Ramos cells. |
| Specificity | Recognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Reacts with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. Tested in flow cytometry on human peripheral blood lymphocytes. Species Crossreactivity: mouse halplotypes b, d and q. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
