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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 2 Antigen DR (H2Ia)

Anti -HLA Class 2 Antigen DR (H2Ia)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Supernatant IH FC
Catalog #H6099-97
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionFlow Cytometry: Neat-1:10. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen, Paraffin.
Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents (Flow Cytometry)F(ab')2 Goat anti-rat IgG: FITC (mouse adsorbed), F(ab')2 Goat anti-rat IgG: RPE (mouse adsorbed)
Recommended Secondary Reagents (Immunohistochemistry)Goat anti-rat IgG: HRP (mouse adsorbed)
Recommended Negative ControlsRat IgG2a Negative Control (for human)
Rat IgG2a Negative Control (for mouse)
HybridomaSpleen cells from immunized AO rats were fused with cells of the rat Y3 Ag1.2.3 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2232
HostRat
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.2M Tris HCl, pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
PurityTissue Culture Supernatant.
ImmunogenEHR-B Ramos cells.
SpecificityRecognizes a monomorphic determinant of human HLA-DR antigen (MHC class II), expressed by B lymphocytes, activated T cells and by monocytes. Reacts with paraffin embedded material following PLP (periodate-lysine-paraformaldehyde) fixation. Tested in flow cytometry on human peripheral blood lymphocytes. Species Crossreactivity: mouse halplotypes b, d and q.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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