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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified IH FC

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.
Catalog #	H6099-97A
Applications	Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Flow Cytometry: 1:50-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
	Immunohistochemistry: frozen tissues
	Immunohistochemistry (paraffin): using PLP (periodate-lysine- paraformaldehyde) fixation
	Optimal dilutions to be determined by researcher.
Recommended Negative Control	I1904-83M1: Rat IgG2a Negative Control
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	3H2232
Host	Rat
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	EHR-B Ramos cells
Specificity	Recognizes a monomorphic determinant of human HLA-DR, expressed by B lymphocytes, monocytes and activated T cells. Reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB (1).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.