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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 2 Antigen DR (H2Ia)

Anti -HLA Class 2 Antigen DR (H2Ia)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR.
Catalog #H6099-97A
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1:50-1:100. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry: frozen tissues
Immunohistochemistry (paraffin): using PLP (periodate-lysine- paraformaldehyde) fixation
Optimal dilutions to be determined by researcher.
Recommended Negative ControlI1904-83M1: Rat IgG2a Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2232
HostRat
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenEHR-B Ramos cells
SpecificityRecognizes a monomorphic determinant of human HLA-DR, expressed by B lymphocytes, monocytes and activated T cells. Reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB (1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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