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You are here:Home » Antibodies » Abs to HLA » Anti -HLA Class 2 Antigen DP, DQ, DR

Anti -HLA Class 2 Antigen DP, DQ, DR

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Catalog #H6099-98C
ApplicationsSuitable for use in Immunohistochemistry (paraffin sections) and Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50-1:200, Use 10ul of the suggested working dilution to label 10 6 cells in 100ul.
Immunohistochemistry: Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH6.0 is recommended.
Optimal working dilutions to be determined by researcher.
HybridomaSpleen cells from immunized BALB/c mice were fused with cells from NS-O mouse myeloma cell line.
Positive ControlTonsil
Negative ControlMouse IgG2a Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H2238
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman HLA Class II (DP, DQ, DR).
SpecificityHLA Class II beta chains of human major histocompatibility antigen Mw 28kD. Reacts with a monomorphic determinant common to each beta chain type. Expressed on human B lymphocytes, monocytes and activated T lymphocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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