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You are here:Home » Antibodies » Antibodies-HLA » Anti -HLA Class 2 Antigen DQ4 (X-Reactive)

Anti -HLA Class 2 Antigen DQ4 (X-Reactive)

Pricing

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Specifications

Clone Host Grade Applications
Mouse Ascites
Catalog #H6100-07
ApplicationsThis antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
Recommended DilutionCytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
Optimal dilutions to be determined by the researcher.
CytotoxicityExpected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
SourceAscites fluid
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Please contact United States Biological for bulk requirements and licensing requests relating to production applications.
Clone Type


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