Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B

4-hydroxy-2-nonenal (4-hydroxynonenal, HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
Catalog #	H6276-10A
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1ug/ml detects HNE in 4-hydroxynonenal or peroxyynitrite-treated recombinant E. coli DNAK
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	9H132
Host	Mouse
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	HNE (4-Hydroxy-2-nonenal) conjugated to KLH
Specificity	Recognizes 4-HNE adducts of histidine residues. It does not cross-react with nitrotyrosine, formaldehyde, glutaraldehyde, or 4-hydroxy hexenaldehyde adducts.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.