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You are here:Home » Antibodies » Abs to Biochemicals » Anti -HNE (4-Hydroxy-2-nonenal)

Anti -HNE (4-Hydroxy-2-nonenal)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
4-hydroxy-2-nonenal (4-hydroxynonenal, HNE) is a highly reactive aldehyde generated by the exposure of polyunsaturated fatty acids to peroxides and reactive oxygen species (ROS). It non-enzymatically forms stable protein adducts with histidine, lysine, and cysteine side chains that have been used as biomarkers for oxidative damage in cells. Conditions where HNE immunoreactivity has been observed include include inflammation, neurodegenerative diseases, and ischemic damage to the heart and brain.
Catalog #H6276-10A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml detects HNE in 4-hydroxynonenal or peroxyynitrite-treated recombinant E. coli DNAK
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No9H132
HostMouse
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenHNE (4-Hydroxy-2-nonenal) conjugated to KLH
SpecificityRecognizes 4-HNE adducts of histidine residues. It does not cross-react with nitrotyrosine, formaldehyde, glutaraldehyde, or 4-hydroxy hexenaldehyde adducts.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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