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H7021 Mouse Anti-HOP, p60 (HSP70 HSP90 Organizing Protein)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Swiss Prot
P31948
Isotype
IgG1
Clone Number
4E142
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Bo Ca Ch Gp Hm Hu Mk Mo Po Rb Rt Sh Xe
Shipping Temp
Blue Ice
Storage Temp
-20°C

Hsp70-Hsp90 Organizing Protein (HOP, p60) is an ~60kD protein that is a critical intermediate component for the efficient maturation of steroid receptor complexes (1–3), serving to recruit Hsp90 to Hsp70-containing complexes. Unactivated steroid hormone receptors are found in hetero-oligomeric complexes that are thought to stabilize a partially folded receptor polypeptide prior to hormone-dependent activation (4–6). Hsp70 and Hsp90 are both known to associate with steroid hormone receptors. Numerous studies (8–13) have shown that Hsp70 functions in an ATP-dependent manner through transient interactions to mediate folding or unfolding of polypeptide chains. Hsp90 is thought to perhaps also function in some capacity related to folding or protein-protein interactions (14). HOP contains three tetratricopeptide repeat (TPR) domains, TPR1, TPR2a and TPR2b. Hsp70 binding has been localized to TPR1, and Hsp90 binding has been localized to TPR2a. Importantly, the highly conserved ñEEVD sequence that terminates many Hsp70 family members, and the similar ñMEEVD sequence that terminates Hsp90, are important recognition sites for the TPR domains. The co-crystal structures for TPR1 plus a GPTIEEVD octapeptide (Hsp70 sequence) and TPR2a plus the MEEVD pentapeptide (Hsp90) have been solved (15). HOP is closely related to a human 63kD protein that is sensitive to simian virus SV 40 transformation (16) and is related to the yeast heat shock-responsive STI1 gene product (17).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot (Colorimetric): 1:1000. Detects a band at ~60kD. Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Chicken HOP (p60)
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.
Purity
Purified by Protein G affinity chromatography from ascites.
Specificity
Recognizes chicken HOP, p60. Species Crossreactivity: human, mouse, rat, bovine, canine, guinea pig, hamster, monkey, mink, porcine, rabbit, sheep and Xenopus.
References
1. Chen, S., et al., J. Biol. Chem. 273: 35,194-35,200 (1998). 2. Dittmar, K.D., et al., J. Biol. Chem. 271: 12,833-12,839 (1996). 3. Kosano, H., et al., J. Biol. Chem. 273: 32,973-32,979 (1998). 4. Smith, D.F., et al., Mol. Cell. Biol. 13: 869-876 (1993). 5. Catelli, M.G., et al., EMBO J. 4: 3131-3135 (1985). 6. Schuh, S., et al., J. Biol. Chem. 260: 14,292-14,296 (1985). 7. Sanchez, E.R., et al., J. Biol. Chem. 260: 12,398-12,401 (1988). 8. Beckmann, R.P., et al., Science 248: 850-854 (1990). 9. Chappell, T.G., et al., Cell 45: 3-12 (1986). 10. Rothman, J.E., Cell 59: 591-601 (1989). 11. Chirico, W.J., et al., Nature (London) 332: 805-810 (1988). 12. Deshaies, R.J., et al., Nature (London) 332: 800-805 (1988). 13. Zimmerman, R.M., et al., EMBO J. 7: 2875-2880 (1988). 14. Ang, D.K., et al., J. Biol. Chem. 266: 24,233-24,236 (1991). 15. Scheufler, C., et al., Cell 101: 199-210 (2000). 16. Honoré, B.H., et al., J. Biol. Chem. 267: 8485-8491 (1992). 17. Nicolet, C.M., et al., Mol. Cell. Biol. 9: 3638-3646 (1989). 18. Nelson, G.M., et al., Cell Stress Chap. 8(2): 125-133 (2003). 19. Prapapanich, V., et al., Mol. Cell. Biol. 18(2): 944-952 (1998). 20. Graumann, K., et al., Biochem. J. 345(3): 627-636 (2000).
USBio References
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