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You are here:Home » Antibodies » Abs to RNA Processing » Anti -HCLS1 associated protein X-1 (HAX1, FLJ17042, FLJ18492, FLJ93803, HAX-1, HCLS1-associated Protein X-1, HCLSBP1, HS1-associating Protein X-1, HS1-binding Protein 1, HS1BP1, SCN3)

Anti -HCLS1 associated protein X-1 (HAX1, FLJ17042, FLJ18492, FLJ93803, HAX-1,
HCLS1-associated Protein X-1, HCLSBP1, HS1-associating Protein X-1, HS1-binding Protein 1,
HS1BP1, SCN3)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene.
Catalog #H7500-18
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 1:2000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See H7500-18-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide C-TRHEADSSPRGDPES, from the internal region of human HAX1.
SpecificityRecognizes human HAX1 at ~33kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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