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You are here:Home » Antibodies » Abs to Heat Shock Proteins » Anti -HSC70-Interacting Protein (HIP)

Anti -HSC70-Interacting Protein (HIP)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit B
Hsc70-interacting protein (Hip) is a cytosolic protein that exists as a homo-oligomer and participates in the regulation of the heat shock protein Hsc70 in eukaryotic cells (1). Hip interacts with the ATPase domain of Hsc70 molecules through its tetratricopeptide repeats and flanking charged alpha-helices (2). Hip and BAG-1 proteins have been shown to compete in binding to the ATPase domain (4). This binding reaction is dependent on Hsp40 in the presence of ATP and becomes Hsp40-independent if Hsc70 is preincubated with ADP (2). Upon binding to Hsc70, Hip stabilizes the ADP-bound state of Hsc70, a conformation that has a high affinity for unfolded substrate proteins. Hip by itself binds unfolded polypeptides, suggesting that it may possess chaperone activity (2,3). Together with Hsp40 and BAG-1, Hip may regulate the chaperone activity of Hsc70 by stabilizing the chaperone-substrate complex.
Catalog #H7651
ApplicationsWestern Blot (Colorimetric): 1:5,000 (mouse, rat)
Western Blot (Colorimetric): 1:1,000 (human, bovine)
Optimal dilutions to be determined by researcher.
Positive Controls
Recombinant Rat Hip Protein
Clone TypePolyclonal
HostRabbit
SourceRat
FormSerum
PuritySerum
ImmunogenRecombinant rat Hip histidine 6 fusion protein.
SpecificityDetects a ~58kD protein, corresponding to the apparent molecular mass of Hip on SDS-PAGE immunoblots, in samples from human, mouse, rat and bovine. The antibody detects both recombinant rat Hip as well as native Hip protein in tissue extracts. Whilst reactivity with human and bovine Hip protein is detected, this reactivity is much weaker than that observed with samples from rat or mouse origin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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