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You are here:Home » Antibodies » Abs to Ubiquitin Ligases » Anti -HSPC150 (UBE2T, Ubiquitin-conjugating Enzyme E2 T, Cell Proliferation-inducing Gene 50 Protein, PIG50, Ubiquitin Carrier Protein T, Ubiquitin-protein Ligase T)

Anti -HSPC150 (UBE2T, Ubiquitin-conjugating Enzyme E2 T, Cell Proliferation-inducing Gene
50 Protein, PIG50, Ubiquitin Carrier Protein T, Ubiquitin-protein Ligase T)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
Catalog #H7681-01C
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Western Blot: 0.1-0.3ug/ml, observed in lysates of cell lines K562, HEK293 and A549 on ~22kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-QLVGIEKKFHPDV, from human UBE2T, at C-terminal (NP_054895.1).
SpecificityRecognizes human UBE2T.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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