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You are here:Home » Biologicals » Cells-Extracts, Lysates » HT29 Cell Lysate

HT29 Cell Lysate

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Specifications

HT29 cells that produce carcinoembryonic antigen (CEA) and are human intestinal epithelial cells which produce the secretory component of immunoglobulin A (IgA). These cells are used for tumorigenicity studies. The lysate is a good positive control for keratin 18, keratin 20, the protooncogene cyclin D1.
Catalog #H7949-25
ApplicationSuitable for Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 10-20ug per lane for mini gel
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceHuman
Concentration~1mg/ml
FormSupplied as a whole lysate in 1 X SDS, 5% b-mercaptoethanol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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