Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Positive Control
Brain
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to amino acids 1844 - 2131 of huntingtin.
Form
Purified IgG - liquid with 0.09% sodium azide.
Purity
Purified IgG.
Specificity
Reacts with an epitope corresponding to the HDB region (amino acids 1844-2131) of the huntingtin protein. Clone HDB4E10 detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. The combined use of clone HDB4E10, HDC8A4 and HDA3E10 demonstrate that huntingtin is enriched in neuronal cells in the brain. Species Crossreactivity: human, rabbit, mouse
References
1. Wilkinson, F. L. et al. (1999). Localization of rabbit huntingtin using a new panel of monoclonal antibodies. Molecular Brain Research. 69: 10-20.