| Huntingtin disease, a neurodegenerate disease, is caused by the expansion of a polymorphic glutamine tract in huntingtin. The Huntingtin Interacting Protein 1 (HIP-1) is a reportedly proapoptotic, cargo-specific adaptor protein that may be involved in the pathogenesis of Huntingtin disease. As well as playing a role in Huntingtin disease, it is likely to be involved in the recruitment of clathrin coats to lipid membranes and it may also factor in tumorigenesis by allowing the survival of precancerous and cancerous cells. Since HIP-1 expression is significantly associated with prostate and colon cancer metastasis, HIP-1 can serve as a putative prognostic factor for prostate and colon cancers. |
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| Catalog # | H7965-03 |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:10,000-1:40,000 |
| Western Blot: 1:500- 1:3000. Detects bands at ~350 and ~200kD. |
| Immunohistochemistry: 1:50- 1:100 |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptides | H7965-03A: Huntington pS421, phosphorylated, CONTROL PEPTIDE (416-424) |
| H7965-03B: Huntingtin Protein, Non-phosphorylated, CONTROL PEPTIDE (416-424) |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, 0.01% sodium azide. No stabilizing proteins added. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A synthetic phosphopeptide corresponding to aa416-424, R-S-R-S-G-pS-I-V-E of the human huntingtin gene protein (mutations cause Huntington's disease poly-GLU expansions causing plaques) |
| Specificity | Recognizes human Huntingtin when phosphorylated at Ser421. Species sequence homology: chimpanzee, porcine and chicken, 100%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
