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You are here:Home » Antibodies » Antibodies-Disease Markers » Anti -Huntingtin Protein, aa549679 (Huntington Disease)

Anti -Huntingtin Protein, aa549679 (Huntington Disease)


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Clone Host Grade Applications
Monoclonal Rat Ascites B IH IC
Catalog #H7965-05
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Not suitable for paraffin. Other applications not tested.
Recommended DilutionWestern Blot: 50ug/lane total protein. Detects a band at 340kD in lymphoblasts from human controls
Immunohistochemistry: 1:1000 3%/0.1% glutaraldehyde paraformaldehyde fixed, frozen tissue, using enzymatic detection
Immunocytochemistry: 3% PFA cells, 5-10 minutes at RT; Permeabilize with Triton X-100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No2Q76
ConcentrationNot Determined
FormSupplied as a liquid, pH 7.2, 0.1% sodium azide.
ImmunogenHuntingtin fusion protein aa 549-679, fused to Glutathione S-tranferase.
SpecificityRecognizes human Huntingtin Protein, residues 549-679.
Species CrossreactivityRat and monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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