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You are here:Home » Antibodies » Abs to Disease Markers » Anti -Huntingtin Protein (Huntington Disease, Htt)

Anti -Huntingtin Protein (Huntington Disease, Htt)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH IC FC
Huntingtin protein (Htt) is a 348kD protein product of Huntington’s disease (HD, IT15) gene. Huntington’s disease is a neurodegenerative disorder caused by a mutation on the HD gene, producing a polyglutamin (polyQ) expansion on the N-terminus of Htt. The mutant Htt leads to cytotoxicity in the striatal neuron (1). This cytotoxicity is modulated by proteolytic cleavage with caspases and calpains, producing N-terminal polyQ fragments. A phosphorylation of Htt may also regulate the cleavage and cytotoxicity of mutant Htt (2). The wild-type Htt up-regulates transcription of brain-derived neurotrophic factor (BDNF), a pro-survival factor for striatal neurons. This suggests restoring wild-type Htt level and increasing BDNF expression level can be therapeutic treatment for Huntington’s disease (3). Huntingtin is also cleaved by apopain, a cysteine protease product of death-gene for human, suggesting HD might be a disorder of inappropriate apoptosis (4).
Catalog #H7965A
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:30
Western Blot: 1:10,000
Immunohistochemistry: 1:250 – 500
Immunocytochemistry: 1:50 – 100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k216
HostRabbit
SourceHuman
FormSupplied as a liquid.
PuritySupernatant
ImmunogenA synthetic peptide corresponding to residues specific to the apopain cleavage site of human huntingtin protein
SpecificityRecognizes human huntingtin protein
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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