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You are here:Home » Molecular Biology » MB-Carbohydrates, Glycoproteins » Hyaluronic Acid, Bovine

Hyaluronic Acid, Bovine

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Specifications

The hyaluronic acids (HA) are a class of macromolecular proteoglycans characterized by a highly polymerized chain of glucuronic acid and N-acetylglucosamine units bonded to protein. They exist in nature as a hydrated gel, usually closely associated with other tissue components such as chrondroitin sulfate. Hyaluronic acid (HA) preparations have variable molecular weights depending on purification procedures, the extent of degradation, as well as the source. The range of molecular weight is 70kD to 2,000-4,000kD in a highly polymerized preparation. Bovine vitreous humor HA has a lower molecular weight than most other sources. The hyaluronic acids are a class of macromolecular proteoglycans characterized by a highly polymerized chain of the repeating disaccharide glucuronic acid -(1-3) N-acetylglucosamine ( 1-4).
Catalog #H7980-11
The molecular weight of HA preparations varies with purification procedures, i.e., the extent of degradation as well as the source. According to Bettelheim (1958) when the protein is completely removed a molecular weight on the order of 70,000 is obtainable as compared with 2-4 million in a highly polymerized preparation (Laurent and Gergely 1955). It has been reported (Laurent et al. 1960) that bovine vitreous humor hyaluronic acid has a lower molecular weight than most other sources and that from human umbilical cord has the largest molecular weight (Jensen and Carlsen 1954). Pigman et al. (1961) report that HA is degraded in the presence of oxygen and reducing agents and by merthiolate, often used in its preparation. Ascorbic acid likewise has this effect (Swann 1967). The carbohydrate polymer is negatively charged. When it is mixed with a cationic protein such as albumin at low pH, a precipitate is formed. If the glycosidic bonds have been split, i.e., the chain depolymerized, this precipitation no longer occurs. This phenomenon is the basis for the turbidimetric assay of hyaluronidase.
Suitable as a substrate for hyaluronidase assays.
SourceBovine vitreous humor
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight80-120kD
SourceBovine vitreous humor
PurityPurified
FormSupplied as a lyophilized powder.
SpecificitySuitable as a substrate for hyaluronidase assays.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCopoly-beta-glucuronic acid; [1-3]-beta-N-acetylglucosamine[1-4] sodium salt


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