Hyaluronic Acid Binding Protein 1, Recombinant, Mouse (Hyaluronan Binding Protein 1,
HABP1, C1qBP, C1q-R, Human complement component 1, q subcomponent binding protein)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3). The 3 kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17). | | | Catalog # | H7980-36 | | A DNA sequence encoding the mature mouse HABP1 (Leu 72-Gln 279; Accession # NP_031599), fused with 2X Met at the N-terminus and a six-histidine tag at the C-terminus, was expressed in E. coli. | | Calculated molecular mass of ~ 24.8kD. | | Activity | Measured by its ability to bind human C1q. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Recombinant Mouse | | Purity | 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/1ug. | | Form | Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
